Journal: International Journal of Molecular Sciences

Article Title: Time Dependent Changes in the Ovine Neurovascular Unit; A Potential Neuroprotective Role of Annexin A1 in Neonatal Hypoxic-Ischemic Encephalopathy

doi: 10.3390/ijms24065929

Figure Lengend Snippet: ( a ) Schematic representation of the NVU including vascular cells (endothelial cells, pericytes), basement membrane, and astrocyte end-foot in the CNS; ( b ) structural illustration of the basement membrane components including laminin and collagen type IV, endothelial cells and the pro-resolving signature of Annexin A1 binding to the formyl peptide receptor 2 (FPR2). Created with Biorender.com .

Article Snippet: The following primary antibodies were administered on tissue slides; polyclonal rabbit ANXA1 (ab137745, Abcam, Cambridge, UK, 1:100), laminin (ab11575, Abcam, 1:100), Collagen IV (ab6586, Abcam; ratio 1:400), and PDGFRβ (#3169, Cell signaling; 1:100) at 4 °C overnight.

Techniques: Binding Assay

Journal: International Journal of Molecular Sciences

Article Title: Time Dependent Changes in the Ovine Neurovascular Unit; A Potential Neuroprotective Role of Annexin A1 in Neonatal Hypoxic-Ischemic Encephalopathy

doi: 10.3390/ijms24065929

Figure Lengend Snippet: ANXA1 and laminin qualitative scoring ( a , c ), and integrated density measurements ( b , d ) on 1, 3 and 7 days after HI insult in the cerebral microvasculature; ( e ) representative images of consecutive sections of cerebral blood vessels stained with ANXA1 and laminin. An average of 40 fields of view per animal were analyzed. Graphs show the average integrated density per field of view (mean grey value of stained area × percentage of stained area) for ANXA1 and laminin expression in coronal brain sections. Statistical analysis was performed with a Kruskal-Wallis test followed by Dunn’s post hoc test. Bars represent mean ± SEM. Scale bar 50 μm. 400× magnification * p < 0.05, ** p < 0.01, # p = 0.06.

Article Snippet: The following primary antibodies were administered on tissue slides; polyclonal rabbit ANXA1 (ab137745, Abcam, Cambridge, UK, 1:100), laminin (ab11575, Abcam, 1:100), Collagen IV (ab6586, Abcam; ratio 1:400), and PDGFRβ (#3169, Cell signaling; 1:100) at 4 °C overnight.

Techniques: Staining, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Time Dependent Changes in the Ovine Neurovascular Unit; A Potential Neuroprotective Role of Annexin A1 in Neonatal Hypoxic-Ischemic Encephalopathy

doi: 10.3390/ijms24065929

Figure Lengend Snippet: Scoring systems of ANXA1, laminin, collagen type IV, and PDGFRβ in cerebral blood vessels (1 = minor, 2 = moderate, 3 = intense immunoreactivity (IR), magnification 400×, scale bar 50 μm).

Article Snippet: The following primary antibodies were administered on tissue slides; polyclonal rabbit ANXA1 (ab137745, Abcam, Cambridge, UK, 1:100), laminin (ab11575, Abcam, 1:100), Collagen IV (ab6586, Abcam; ratio 1:400), and PDGFRβ (#3169, Cell signaling; 1:100) at 4 °C overnight.

Techniques: